Journal: Hormones and behavior

Article Title: Oxytocin promotes functional coupling between paraventricular nucleus and both sympathetic and parasympathetic cardioregulatory nuclei

doi: 10.1016/j.yhbeh.2016.01.010

Figure Lengend Snippet: Functional coupling of PVN and brainstem cardioregulatory regions by OXT administration (as shown in Fig. 4) may not involve OXT-secreting neurons in the PVN. (Top) Representative photomicrograph of OXT-ir/Fos-ir double-immunolabeling as analyzed with the TissueFAXS/HistoQuest cell sorting system which accurately sorts cells by chromogen. (Bottom) Correlation matrix depicting associations between percentage of OXT-ir/Fos-ir double-immunolabeled cells in the PVN (abscissa, all graphs), and Fos-ir in the dorsal vagal complex (DVC; ordinate, top row), nucleus ambiguous (AMB; ordinate, middle row), and rostral ventrolateral medulla (RVLM; ordinate, bottom row). Saline controls (SAL) are represented in the first column, OXT-treated voles are represented in the second column, and OXT-A-treated voles are represented in the third column.

Article Snippet: For quantification of double-labeled OXT-ir/Fos-ir in the PVN-H, images were acquired using a Zeiss AXIO Imager Z1 (Zeiss, Oberkochen, Germany) and counted using the TissueFAXS Acquisition Software with HistoQuest Cell Analysis Module (Tissue Gnostics, Vienna, Austria).

Techniques: Functional Assay, Immunolabeling, FACS